Department of Analytical Biochemistry and Interfaculty Mass Spectrometry Center, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands.
Mass Spectrom Rev. 2023 Mar;42(2):546-576. doi: 10.1002/mas.21709. Epub 2021 Jun 6.
Advancements in liquid chromatography and mass spectrometry over the last decades have led to a significant development in mass spectrometry-based proteome quantification approaches. An increasingly attractive strategy is multiplex isotope labeling, which significantly improves the accuracy, precision and throughput of quantitative proteomics in the data-dependent acquisition mode. Isotope labeling-based approaches can be classified into MS1-based and MS2-based quantification. In this review, we give an overview of approaches based on chemical isotope labeling and discuss their principles, benefits, and limitations with the goal to give insights into fundamental questions and provide a useful reference for choosing a method for quantitative proteomics. As a perspective, we discuss the current possibilities and limitations of multiplex, isotope labeling approaches for the data-independent acquisition mode, which is increasing in popularity.
过去几十年中，液相色谱和质谱技术的进步使得基于质谱的蛋白质组定量方法得到了显著发展。一种越来越有吸引力的策略是多重同位素标记，它显著提高了数据依赖采集模式下定量蛋白质组学的准确性、精密度和通量。基于同位素标记的方法可分为基于 MS1 和基于 MS2 的定量。在本文中，我们综述了基于化学同位素标记的方法，并讨论了它们的原理、优点和局限性，以期深入了解基本问题，并为定量蛋白质组学选择方法提供有用的参考。作为一个展望，我们讨论了当前多重、同位素标记方法在数据非依赖采集模式下的可能性和局限性，这种模式越来越受欢迎。
Zotero 插件
