去污剂重新激活的干扰素未复性的证据。
Evidence that detergent-reactivated interferons are not renatured.
作者信息
Allen P T, Stewart W E
出版信息
J Gen Virol. 1976 Jul;32(1):133-7. doi: 10.1099/0022-1317-32-1-133.
PMID:182910
Abstract
The sedimentation rate of human leukocyte interferon (HLIF) reactivated from sodium dodecyl sulphate (SDS) solution was studied by glycerol gradient centrifugation and compared to that of native HLIF. Reactivated HLIF consistently sedimented faster than native HLIF, indicating that full recovery of antiviral activity does not require renaturation of the entire interferon molecule.
摘要
通过甘油梯度离心法研究了从十二烷基硫酸钠（SDS）溶液中重新激活的人白细胞干扰素（HLIF）的沉降速率，并与天然HLIF的沉降速率进行了比较。重新激活的HLIF沉降速度始终比天然HLIF快，这表明抗病毒活性的完全恢复并不需要整个干扰素分子复性。
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