用溶液杂交/保护分析法检测大鼠中多巴胺D2受体mRNA剪接变体的分布。
Distribution of dopamine D2 receptor mRNA splice variants in the rat by solution hybridization/protection assay.
作者信息
Snyder L A, Roberts J L, Sealfon S C
机构信息
Fishberg Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029.
出版信息
Neurosci Lett. 1991 Jan 14;122(1):37-40. doi: 10.1016/0304-3940(91)90187-x.
PMID:1829147
Abstract
We investigated the distribution of the two dopamine D2 receptor mRNA splice variants in the rat using a sensitive and quantitative solution hybridization/nuclease protection assay. In all brain and endocrine regions studied, both splice variants were detected and the mRNA of the longer form (D2L) was more abundant than that of the shorter form (D2S). The lowest percentages of D2S were found in the pituitary and adrenal glands.
摘要
我们使用灵敏且定量的溶液杂交/核酸酶保护分析法，研究了两种多巴胺D2受体mRNA剪接变体在大鼠体内的分布情况。在所研究的所有脑区和内分泌区域中，均检测到了这两种剪接变体，且较长形式（D2L）的mRNA比短形式（D2S）更为丰富。垂体和肾上腺中D2S的比例最低。
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