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通过噬菌体展示技术制备抗B型肉毒杆菌神经毒素轻链单克隆抗体及其特性分析

Production and characterization of monoclonal antibody to botulinum neurotoxin type B light chain by phage display.

作者信息

Lee Myung-Shin, Lee Ji Chul, Choi Cha Yong, Chung Junho

机构信息

Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon, South Korea.

出版信息

Hybridoma (Larchmt). 2008 Feb;27(1):18-24. doi: 10.1089/hyb.2007.0532.

DOI:10.1089/hyb.2007.0532
PMID:18294072
Abstract

A monoclonal antibody to the light chain of botulinum neurotoxin type B (BoNT/B) was generated and its protective activity was evaluated in vivo. A chimeric rabbit/human Fab library was generated using bone marrow and spleen cDNAs of rabbits immunized with the BoNT/B light chain, and three monoclonal antibodies specific to the catalytic domain of BoNT/B were isolated. One of these clones, BCXRH1, was specific to a conformation-dependent epitope, and partially neutralized the BoNT/B complex in vivo.

摘要

制备了一种针对B型肉毒杆菌神经毒素（BoNT/B）轻链的单克隆抗体，并在体内评估了其保护活性。利用免疫了BoNT/B轻链的兔的骨髓和脾脏cDNA构建了一个嵌合兔/人Fab文库，并分离出三种针对BoNT/B催化结构域的单克隆抗体。其中一个克隆BCXRH1对一个构象依赖性表位具有特异性，并在体内部分中和了BoNT/B复合物。

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