Can Ismail, Tahara-Hanaoka Satoko, Shibuya Akira
Department of Immunology, Institute of Basic Medical Sciences and Center for TARA, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
Hybridoma (Larchmt). 2008 Feb;27(1):59-61. doi: 10.1089/hyb.2007.0534.
The myeloid-associated immunoglobulin-like receptor (MAIR) family consists of nine activating or inhibitory cell surface receptors preferentially expressed on myeloid cells, which are encoded by the genes in a small cluster on mouse chromosome 11. MAIR-Va, one of the inhibitory receptors in the family, has a splicing isoform, MAIR-Vb. However, the expression profile of MAIR-Va and MAIR-Vb has remained unclear. Here, we generated three monoclonal antibodies (MAb)-TX70, TX71, and TX73-against MAIR-V. TX70 and TX73 MAbs recognized both MAIR-Va and MAIR-Vb. In contrast, TX71 recognized MAIR-Va, but not MAIR-Vb. Western blot analyses by using these MAbs revealed that MAIR-Va and MAIR-Vb were almost equally expressed in the spleen cells and peritoneal exudate cells. Thus, these MAbs are useful for analyses of molecular and functional characteristics of MAIR-Va and MAIR-Vb.
髓系相关免疫球蛋白样受体（MAIR）家族由九个激活或抑制性细胞表面受体组成，这些受体优先在髓系细胞上表达，由小鼠11号染色体上一个小簇中的基因编码。MAIR-Va是该家族中的抑制性受体之一，有一个剪接异构体MAIR-Vb。然而，MAIR-Va和MAIR-Vb的表达谱仍不清楚。在此，我们制备了三种针对MAIR-V的单克隆抗体（MAb）——TX70、TX71和TX73。TX70和TX73单克隆抗体识别MAIR-Va和MAIR-Vb两者。相反，TX71识别MAIR-Va，但不识别MAIR-Vb。使用这些单克隆抗体进行的蛋白质免疫印迹分析表明，MAIR-Va和MAIR-Vb在脾细胞和腹腔渗出细胞中几乎等量表达。因此，这些单克隆抗体可用于分析MAIR-Va和MAIR-Vb的分子和功能特性。
