Prammananan T, Cheunoy W, Taechamahapun D, Yorsangsukkamol J, Phunpruch S, Phdarat P, Leechawengwong M, Chaiprasert A
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand.
Clin Microbiol Infect. 2008 May;14(5):446-53. doi: 10.1111/j.1469-0691.2008.01951.x. Epub 2008 Feb 22.
Since rifampicin resistance is a surrogate marker for multidrug-resistant Mycobacterium tuberculosis (MDRTB), the present study aimed to investigate rpoB mutations conferring rifampicin resistance in M. tuberculosis strains from Thailand, and to develop a rapid, inexpensive and simple PCR-based method for rapid detection of MDRTB. Overall, 267 M. tuberculosis isolates, including 143 MDRTB isolates, were investigated. Isolates of the Beijing strain predominated among the MDRTB isolates (79.1%), but accounted for only 45.5% of the susceptible isolates. Mutations in the rpoB gene were found most commonly at codons 531, 526 and 516 (58%, 25.2% and 9.1%, respectively). A multiplex allele-specific PCR was developed and tested with 216 clinical isolates. In comparison with the proportion method, the method showed 94.2% sensitivity and 100% specificity, and had a 100% positive predictive value and a 95% negative predictive value, which suggested that this method could be useful for screening for MDRTB, particularly in resource-limited countries.
由于利福平耐药是耐多药结核分枝杆菌（MDRTB）的替代标志物，本研究旨在调查泰国结核分枝杆菌菌株中赋予利福平耐药性的rpoB基因突变，并开发一种基于PCR的快速、廉价且简单的方法用于快速检测MDRTB。总体而言，共调查了267株结核分枝杆菌分离株，其中包括143株MDRTB分离株。北京菌株的分离株在MDRTB分离株中占主导地位（79.1%），但在敏感分离株中仅占45.5%。rpoB基因的突变最常见于密码子531、526和516（分别为58%、25.2%和9.1%）。开发了一种多重等位基因特异性PCR，并对216株临床分离株进行了测试。与比例法相比，该方法的灵敏度为94.2%，特异性为100%，阳性预测值为100%，阴性预测值为95%，这表明该方法可用于筛查MDRTB，特别是在资源有限的国家。
