Sakamoto Yuichi, Ishikawa Tomomoto, Kondo Yutaka, Yamaguchi Kohei, Fujisawa Masato
Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
BJU Int. 2008 Jun;101(12):1547-52. doi: 10.1111/j.1464-410X.2008.07517.x. Epub 2008 Feb 21.
To assess oxidative stress markers, antioxidant capacity and cytokines in seminal plasma from infertile patients with varicocele, and to investigate seminal oxidative status and sperm DNA damage after varicocelectomy.
PATIENTS, SUBJECTS AND METHODS: The records were retrospectively evaluated for 28 azoospermic, 30 oligospermic (15 with varicocele and 15 without) and 30 patients with normal semen characteristics (15 with varicocele and 15 without). The mean (sd) age of the men was 32.4 (5.6) years; all men with varicocele had a unilateral or bilateral microsurgical subinguinal varicocelectomy. The level of nitric oxide (NO), 8-hydroxy-2'-deoxyguanosine (8-OHdG), hexanoyl-lysine (HEL), superoxide dismutase (SOD) activity, interleukin (IL)-6, IL-8 and tumour necrosis factor-alpha in seminal plasma were measured. In addition, sperm DNA fragmentation was analysed before and 6 months after varicocelectomy.
Azoospermic and oligospermic patients had a significantly higher HEL concentration and SOD activity in seminal plasma; those with varicocele had a significantly higher NO, HEL, and SOD activity in seminal plasma. There was a significant increase in sperm concentration and reduction in NO, HEL, 8-OHdG level and SOD activity after varicocelectomy. Oligospermic patients with varicocele had a significantly higher IL-6 level in seminal plasma, and there was a significant reduction after varicocelectomy. The percentage of apoptosis-positive sperm decreased significantly after varicocelectomy.
This study indicates that the seminal plasma of patients with varicocele is under excessive oxidative stress, and partly even in patients with normospermia, and that varicocelectomy reduces oxidative stress in seminal plasma and ameliorates sperm DNA damage.
评估精索静脉曲张不育患者精液中的氧化应激标志物、抗氧化能力和细胞因子，并研究精索静脉结扎术后精液氧化状态和精子DNA损伤情况。
患者、研究对象与方法：回顾性评估28例无精子症患者、30例少精子症患者（其中15例有精索静脉曲张，15例无精索静脉曲张）以及30例精液特征正常患者（其中15例有精索静脉曲张，15例无精索静脉曲张）的记录。男性平均（标准差）年龄为32.4（5.6）岁；所有精索静脉曲张患者均接受了单侧或双侧显微腹股沟下精索静脉结扎术。测量精液中一氧化氮（NO）、8-羟基-2'-脱氧鸟苷（8-OHdG）、己酰赖氨酸（HEL）、超氧化物歧化酶（SOD）活性、白细胞介素（IL）-6、IL-8和肿瘤坏死因子-α水平。此外，在精索静脉结扎术前和术后6个月分析精子DNA片段化情况。
无精子症和少精子症患者精液中HEL浓度和SOD活性显著更高；精索静脉曲张患者精液中NO、HEL和SOD活性显著更高。精索静脉结扎术后精子浓度显著增加，NO、HEL、8-OHdG水平和SOD活性降低。精索静脉曲张少精子症患者精液中IL-6水平显著更高，精索静脉结扎术后显著降低。精索静脉结扎术后凋亡阳性精子百分比显著降低。
本研究表明，精索静脉曲张患者的精液处于过度氧化应激状态，部分正常精子症患者亦是如此，精索静脉结扎术可降低精液中的氧化应激并改善精子DNA损伤。
