Ren J
Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1991 Mar;66(2):187-200.
The present study was undertaken to elucidate the ultrastructural differences between tumor cell clones with high growth or highly metastatic potential and those with low growth or weakly metastatic potential. Transmission, scanning and immuno-electron microscopy revealed a remarkable variance of surface microvilli between these two types of tumor cell clones. Greater numbers of microvilli appeared on the tumor cells which possess high growth or highly metastatic potential than on those of tumor cells with low growth or weakly metastatic potential. In contrast, gap junctions and desmosomes were more occasionally seen on the weakly metastatic tumor cells than the highly metastatic tumor cells. Immuno-electron microscopy revealed that epidermal growth factor (EGF) receptor and c-neu oncogene product, which is closely related to the EGF-receptor, were positively stained on the microvilli of tumor cells with high growth potential, whereas the tumor cells with low growth potential showed almost no staining. Western blot analysis also revealed that in the tumor cells with high growth potential, positive expression of c-neu at 185 kDa was stronger than in the tumor cells with low growth potential. The findings suggest that increased numbers of microvilli are closely related to the growth and metastatic potential of tumor cells.
本研究旨在阐明具有高生长或高转移潜能的肿瘤细胞克隆与低生长或低转移潜能的肿瘤细胞克隆之间的超微结构差异。透射电子显微镜、扫描电子显微镜和免疫电子显微镜显示，这两种类型的肿瘤细胞克隆之间表面微绒毛存在显著差异。具有高生长或高转移潜能的肿瘤细胞上出现的微绒毛数量多于低生长或低转移潜能的肿瘤细胞。相反，低转移潜能的肿瘤细胞比高转移潜能的肿瘤细胞更偶尔可见缝隙连接和桥粒。免疫电子显微镜显示，表皮生长因子（EGF）受体和与EGF受体密切相关的c-neu癌基因产物在具有高生长潜能的肿瘤细胞微绒毛上呈阳性染色，而低生长潜能的肿瘤细胞几乎没有染色。蛋白质免疫印迹分析还显示，在具有高生长潜能的肿瘤细胞中，185 kDa的c-neu阳性表达比低生长潜能的肿瘤细胞更强。这些发现表明，微绒毛数量的增加与肿瘤细胞的生长和转移潜能密切相关。
