Ren J, Hamada J, Takeichi N, Fujikawa S, Kobayashi H
Laboratory of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Cancer Res. 1990 Jan 15;50(2):358-62.
We examined by electron microscopy the differences in junctional intercellular communications among highly metastatic clones, weakly metastatic clones, and the parent clone obtained from a spontaneously developed rat mammary carcinoma. We also investigated intercellular communications of the highly and weakly metastatic clone cells with normal fibroblasts. The results showed that ultrastructural changes of the highly metastatic clone cells, such as microvilli, microfilaments, and small organelles including endoplasmic reticulum, Golgi apparatus, and mucous particles, were more distinct than those of the weakly metastatic clone cells, and that the numbers of desmosome and gap junctions of weakly metastatic clone cells were significantly greater than those of highly metastatic clone cells. The formation of gap junctions and desmosomes was found only between weakly metastatic clone cells and normal fibroblasts. When both highly and weakly metastatic clone cells were cultured with normal fibroblasts, a tight junction was observed only in the culture of weakly metastatic clone cells and normal fibroblasts. These results suggest that ultrastructural differences are related to the proliferation and detachment of tumor cells from the primary site in the initial stage of tumor metastasis.
我们通过电子显微镜检查了从自发形成的大鼠乳腺癌中获得的高转移克隆、低转移克隆和亲本克隆之间的连接性细胞间通讯差异。我们还研究了高转移克隆细胞和低转移克隆细胞与正常成纤维细胞之间的细胞间通讯。结果显示，高转移克隆细胞的超微结构变化，如微绒毛、微丝以及包括内质网、高尔基体和黏液颗粒在内的小细胞器，比低转移克隆细胞的更为明显，并且低转移克隆细胞的桥粒和间隙连接数量显著多于高转移克隆细胞。仅在低转移克隆细胞与正常成纤维细胞之间发现了间隙连接和桥粒的形成。当高转移克隆细胞和低转移克隆细胞都与正常成纤维细胞共培养时，仅在低转移克隆细胞与正常成纤维细胞的培养物中观察到紧密连接。这些结果表明，超微结构差异与肿瘤转移初始阶段肿瘤细胞从原发部位的增殖和脱离有关。
