Tartivita K A, Sciarello J P, Rudy B C
J Pharm Sci. 1976 Jul;65(7):1024-7. doi: 10.1002/jps.2600650717.
A high-performance liquid chromatographic (HPLC) procedure was developed for the quantitative determination of cholecalciferol or ergocalciferol in the presence of the photochemical isomers of the provitamin. Separation is also achieved from various common reaction products encountered in the vitamin synthesis as well as other fat-soluble vitamins. The method was applied to the routine analysis of cholecalciferol resins, and experimental data are set forth. A comparison of the HPLC method to the AOAC biological and chemical procedures shows that the HPLC method most closely approximates the antirachitic activity of a cholecalciferol sample. The specificity, sensitivity, and reproducibility of the method make it applicable to various vitamin samples containing cholecalciferol or ergocalciferol.
开发了一种高效液相色谱（HPLC）方法，用于在维生素原的光化学异构体存在下定量测定胆钙化醇或麦角钙化醇。还可从维生素合成中遇到的各种常见反应产物以及其他脂溶性维生素中实现分离。该方法应用于胆钙化醇树脂的常规分析，并列出了实验数据。将HPLC方法与AOAC生物和化学方法进行比较表明，HPLC方法最接近胆钙化醇样品的抗佝偻病活性。该方法的特异性、灵敏度和重现性使其适用于各种含有胆钙化醇或麦角钙化醇的维生素样品。
