Hofsass H, Grant A, Alicino N J, Greenbaum S B
J Assoc Off Anal Chem. 1976 Mar;59(2):251-60.
A high-pressur liquid chromatographic (HPLC) procedure has been developed for the determination of vitamin D3 in resins, oils, dry concentrates, and dry concentrates containing vitamin A. Specificity of the method for vitamin D3 in the presence of isomers and other related constituents was shown by ultimate recovery of the vitamin D3 and the individual constituents and their characterization by other methods such as ultraviolet spectrophotometry. Precision and accuracy are within 2%, and as many as 20 determinations may be completed in a working day. Excellent agreement with the official AOAC biological assay was found. A comparison of the response of isomers of vitamin D3 by the HPLC method vs. other instrumental and chemical procedures shows HPLC to be the most specific method for determining the bioactive isomers.
已开发出一种高压液相色谱（HPLC）方法，用于测定树脂、油类、干浓缩物以及含维生素A的干浓缩物中的维生素D3。通过维生素D3及各成分的最终回收率以及用紫外分光光度法等其他方法对其进行表征，证明了该方法在存在异构体和其他相关成分时对维生素D3的特异性。精密度和准确度在2%以内，一个工作日内可完成多达20次测定。发现与官方AOAC生物测定法的结果高度一致。通过HPLC方法与其他仪器和化学方法对维生素D3异构体的响应进行比较，结果表明HPLC是测定生物活性异构体最具特异性的方法。
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