Benkovic Stephen J, Hammes Gordon G, Hammes-Schiffer Sharon
Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Biochemistry. 2008 Mar 18;47(11):3317-21. doi: 10.1021/bi800049z. Epub 2008 Feb 26.
The concept is developed that enzyme mechanisms should be viewed as "catalytic networks" with multiple conformations that occur serially and in parallel in the mechanism. These coupled ensembles of conformations require a multi-dimensional standard free-energy surface that is very "rugged", containing multiple minima and transition states. Experimental and theoretical evidence is presented to support this concept.
提出了这样一种概念，即酶机制应被视为“催化网络”，在该机制中存在多个依次和平行出现的构象。这些构象的耦合集合需要一个非常“崎岖不平”的多维标准自由能表面，其中包含多个最小值和过渡态。文中给出了实验和理论证据来支持这一概念。
