Rajkumar Sankaranarayanan, Praveen Mamidipudi R, Gajjar Devarshi, Vasavada Abhay R, Alapure Bhagwat, Patel Dhara, Kapur Suman
Iladevi Cataract & IOL Research Centre, Raghudeep Eye Clinic, Ahmedabad, India.
J Cataract Refract Surg. 2008 Mar;34(3):470-4. doi: 10.1016/j.jcrs.2007.10.044.
To evaluate the level of superoxide dismutase (SOD) and SOD isoenzyme activity in lens epithelial cells (LECs) derived from different types of cataract in patients having phacoemulsification.
Iladevi Cataract & IOL Research Centre, Memnagar, Ahmedabad, India.
This observational study of 109 patients having phacoemulsification was performed to evaluate the level of activity in LECs of total superoxide dismutase (TSOD) and 2 superoxide dismutase isoenzymes: copper- (Cu) and zinc (Zn)-dependent SOD (Cu/Zn-SOD) and manganese (Mn)-dependent SOD (Mn-SOD). The curvilinear capsulorhexis (lens capsule) harboring LECs was obtained during phacoemulsification. The anterior lens capsule samples were processed for assaying SOD activity using the nitro blue tetrazolium reduction assay. The samples were grouped based on age and on pure cataract types.
The highest level of TSOD, Cu/Zn-SOD, and Mn-SOD activity was in patients 50 years or younger. The activity declined gradually with age (P<.001). The level of TSOD activity increased in cortical cataract. The level of Cu/Zn-SOD and Mn-SOD isoenzyme activity in LECs was higher in cortical cataracts. A significant difference in the level of TSOD and Cu/Zn-SOD activity was found with different types of cataract (P<.001).
The level of TSOD, Cu/Zn-SOD, and Mn SOD isoenzyme activity decreased with age in LECs of patients with age-related cataract. A significant difference in the level of TSOD and Cu/Zn-SOD isoenzyme activity between different types of cataract was observed. The activity of all 3 SOD isoenzymes was highest in cortical cataracts.
评估接受超声乳化白内障吸除术患者不同类型白内障来源的晶状体上皮细胞（LECs）中超氧化物歧化酶（SOD）水平及SOD同工酶活性。
印度艾哈迈达巴德梅姆纳加尔伊拉德维白内障与人工晶状体研究中心。
对109例接受超声乳化白内障吸除术的患者进行观察性研究，以评估总超氧化物歧化酶（TSOD）以及2种超氧化物歧化酶同工酶：铜（Cu）和锌（Zn）依赖性SOD（Cu/Zn-SOD）和锰（Mn）依赖性SOD（Mn-SOD）在LECs中的活性水平。在超声乳化白内障吸除术中获取含有LECs的连续环形撕囊（晶状体囊膜）。对晶状体前囊膜样本进行处理，采用氮蓝四唑还原法测定SOD活性。样本根据年龄和单纯白内障类型进行分组。
TSOD、Cu/Zn-SOD和Mn-SOD活性最高的是50岁及以下患者。活性随年龄逐渐下降（P<0.001）。皮质性白内障患者的TSOD活性水平升高。皮质性白内障中LECs的Cu/Zn-SOD和Mn-SOD同工酶活性水平较高。不同类型白内障的TSOD和Cu/Zn-SOD活性水平存在显著差异（P<0.001）。
年龄相关性白内障患者LECs中TSOD、Cu/Zn-SOD和Mn SOD同工酶活性随年龄下降。不同类型白内障之间TSOD和Cu/Zn-SOD同工酶活性水平存在显著差异。所有3种SOD同工酶的活性在皮质性白内障中最高。
