Jimenez-Coello Matilde, Vado-Solis Ignacio, Cárdenas-Marrufo Maria F, Rodríguez-Buenfil Jorge C, Ortega-Pacheco Antonio
Departamento de Biomedicina de Enfermedades Infecciosas y Parasitarias, Laboratorio de Biología Celular, CIR Hideyo Noguchi, Av. Itzaes #490, AP. 4713, Mérida Yucatán, Mexico.
Acta Trop. 2008 Apr;106(1):22-6. doi: 10.1016/j.actatropica.2007.12.011. Epub 2008 Jan 5.
Blood samples were taken from 400 stray dogs. The microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA) test were implemented using Leptospira interrogans serogroups canicola, hardjo, pyrogenes, panama, pomona, tarassovi, icterohaemorrhagiae, gryppotyphosa, wolffi and brastislava. For the ELISA test, sonicated antigen from above mentioned cultures was used. A conjugate AP-labeled anti-dog IgG antibody was used, the optimal cut-off point of ELISA was set at 1.34. Concordance between ELISA and MAT titers was measured by kappa (kappa). Overall prevalence was 35%. The most prevalent serogroups were canicola and icterohaemorrhagiae. Positive samples showed titers between 1:100 and 1:25,600, with higher titers found in serogroups canicola and icterohaemorrhagiae. Positive serum samples fell within a range of 1.36-1.65. A correlation index of 96% was found between MAT and ELISA. The sensitivity of ELISA was 98.6% and specificity 95.8%. Seroprevalence of canine leptospirosis and titers were high as a direct consequence of environmental conditions in the studied area. The ELISA test showed a good sensitivity, resulting in a good alternative test for the detection of leptospiral antibodies in dog serum.
从400只流浪狗身上采集血样。使用问号钩端螺旋体犬型、哈焦型、致热型、巴拿马型、波摩那型、塔拉索夫型、出血性黄疸型、犬伤寒型、沃尔夫型和布拉迪斯拉发型血清群进行显微镜凝集试验（MAT）和酶联免疫吸附测定（ELISA）检测。对于ELISA检测，使用上述培养物的超声处理抗原。使用结合了碱性磷酸酶（AP）标记的抗犬IgG抗体，将ELISA的最佳临界值设定为1.34。通过kappa（κ）系数测量ELISA和MAT滴度之间的一致性。总体患病率为35%。最常见的血清群是犬型和出血性黄疸型。阳性样本的滴度在1:100至1:25,600之间，犬型和出血性黄疸型血清群中发现的滴度更高。阳性血清样本在1.36 - 1.65范围内。MAT和ELISA之间的相关指数为96%．ELISA的敏感性为98.6%，特异性为95.8%。由于研究区域的环境条件，犬钩端螺旋体病的血清阳性率和滴度很高。ELISA检测显示出良好的敏感性，是检测犬血清中钩端螺旋体抗体的良好替代检测方法。
