Felt Stephen A, Wasfy Momtaz O, El-Tras Wael F, Samir Ahmed, Rahaman Bassem Abdel, Boshra Marie, Parker Tina M, Hatem Mahmoud Essam, El-Bassiouny Ahmed Ahmed, Murray Clinton K, Pimentel Guillermo
Laboratory Unit, United States Naval Medical Research Unit-3, Imtidad Ramses Street, Abbassia, Cairo, Egypt.
Am J Trop Med Hyg. 2011 Mar;84(3):420-5. doi: 10.4269/ajtmh.2011.10-0393.
A survey of 179 animals (black rats, dogs, sheep, buffaloes, cattle, donkeys, weasels, and cats) for Leptospira infection was conducted in Mahalla City (Lower Egypt). Blood, urine, and kidney were collected and tested by culture, microscopic agglutination test (MAT), and/or polymerase chain reaction (PCR). Among rats, 26% were positive by PCR, including 7% that were also positive by culture for L. interrogans serovars Grippotyphosa, Pyrogenes, and Icterohaemorrhagiae. L. borpetersenii serovar Polonica was isolated for the first time in Egypt in three rats. MAT titers ≥ 1:800 were observed in 11% of rats and 12% of dogs. L. interrogans serovar Grippotyphosa was detected in one cat. Sheep and donkeys were negative for leptospirosis by all methods. Buffaloes and cattle were seropositive in 20% and 44% of animals, respectively. Data indicate that several pathogenic serovars are circulating in the animals, which may pose exposure risks and account for high rates of acute febrile illness.
在埃及下埃及的迈哈莱市，对179只动物（黑鼠、狗、绵羊、水牛、牛、驴、黄鼬和猫）进行了钩端螺旋体感染调查。采集了血液、尿液和肾脏样本，并通过培养、显微镜凝集试验（MAT）和/或聚合酶链反应（PCR）进行检测。在大鼠中，26%通过PCR呈阳性，其中7%通过培养也呈阳性，感染的是问号钩端螺旋体血清型波摩那型、热解型和出血黄疸型。在三只大鼠中首次在埃及分离出波摩那钩端螺旋体血清型波兰型。在11%的大鼠和12%的狗中观察到MAT效价≥1:800。在一只猫中检测到问号钩端螺旋体血清型波摩那型。绵羊和驴通过所有方法检测钩端螺旋体病均为阴性。水牛和牛的血清阳性率分别为20%和44%。数据表明，几种致病性血清型在动物中传播，这可能带来接触风险，并导致急性发热性疾病的高发病率。
