Kelly Terri-Ann N, Fisher Matthew B, Oswald Elizabeth S, Tai Timon, Mauck Robert L, Ateshian Gerard A, Hung Clark T
Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, MC 8904, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Ann Biomed Eng. 2008 May;36(5):769-79. doi: 10.1007/s10439-008-9476-1. Epub 2008 Feb 26.
High-serum media have been shown to produce significant improvement in the properties of tissue-engineered articular cartilage when applied in combination with dynamic deformational loading. To mitigate concerns regarding the culture variability introduced by serum, we examined the interplay between low-serum/ITS-supplemented media and dynamic deformational loading. Our results show that low serum/ITS supplementation does not support the same level of tissue formation as compared to high serum controls. In free-swelling culture, using a combination of ITS with concentrations of FBS above 2% negated the beneficial effects of ITS. Although there were beneficial effects with loading and 0.2%FBS + ITS, these constructs significantly underperformed relative to 20%FBS constructs. At 2%FBS + ITS, the free-swelling construct stiffness and composition approached or exceeded that of 20%FBS constructs. With dynamic loading, the properties of 2%FBS + ITS constructs were significantly lower than free-swelling controls and 20%FBS constructs by day 42. By priming the chondrocytes in 20%FBS prior to exposure to low-serum/ITS media, we observed that low-serum/ITS media produced significant enhancement in tissue properties compared to constructs grown continuously in 20%FBS.
高血清培养基与动态变形加载联合应用时，已显示出能显著改善组织工程关节软骨的性能。为减轻对血清引入的培养变异性的担忧，我们研究了低血清/ITS补充培养基与动态变形加载之间的相互作用。我们的结果表明，与高血清对照组相比，低血清/ITS补充不能支持相同水平的组织形成。在自由膨胀培养中，将ITS与高于2%的胎牛血清（FBS）浓度组合使用会抵消ITS的有益作用。尽管加载和0.2%FBS + ITS有有益效果，但这些构建体相对于20%FBS构建体表现明显较差。在2%FBS + ITS时，自由膨胀构建体的硬度和组成接近或超过20%FBS构建体。动态加载时，到第42天，2%FBS + ITS构建体的性能显著低于自由膨胀对照组和20%FBS构建体。通过在暴露于低血清/ITS培养基之前用20%FBS预处理软骨细胞，我们观察到与在20%FBS中连续培养的构建体相比，低血清/ITS培养基能显著提高组织性能。
