乙基甲烷磺酸盐和甲基甲烷磺酸盐对T7噬菌体中DNA注入及基因重组的作用。
Action of ethyl and methyl methane sulfonates on DNA injection and genetic recombination in T7 bacteriophage.
作者信息
Karska-Wysocki B, Mamet-Bratley M D, Verly W G
出版信息
J Virol. 1976 Aug;19(2):318-24. doi: 10.1128/JVI.19.2.318-324.1976.
Abstract
After treatment with methyl or ethyl methane sulfonate, T7 amber mutants display a reduced capacity for recombination. Moreover, alkylation reduces recombination frequency involving markers on the right-hand side of the genetic map more than it reduces recombination frequency involving markers on the left-hand side. We interpret this to mean that alkylation can stop DNA injection at any point along the DNA molecule, and that T7 phage injects its DNA in a unique fashion starting from the end carrying the genes for early proteins.
摘要
用甲基磺酸甲酯或甲基磺酸乙酯处理后，T7琥珀突变体的重组能力降低。此外，烷基化对遗传图谱右侧标记的重组频率的降低作用比对左侧标记的重组频率的降低作用更大。我们认为这意味着烷基化可在DNA分子的任何位置阻止DNA注入，并且T7噬菌体以独特的方式从携带早期蛋白基因的末端开始注入其DNA。
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