Hartman P S, Einsenstark A
J Virol. 1982 Aug;43(2):529-32. doi: 10.1128/JVI.43.2.529-532.1982.
Near-UV (NUV) (300 to 400 nm) and far-UV (FUV) (254 nm) radiations damage bacteriophage by different mechanisms. Host cell reactivation, Weigle reactivation, and multiplicity reactivation were observed upon FUV, but not upon NUV irradiation. Also, the number of his+ recombinants increased with P22 bacteriophage transduction in Salmonella typhimurium after FUV, but not after NUV irradiation. This loss of reactivation and recombination after NUV irradiation was not necessarily due to host incapability to repair phage damage. Instead, the phage genome failed to enter the host cell after NUV irradiation. In the case of NUV-irradiated T7 phage, this was determined by genetic crosses with amber mutants, which demonstrated that either "all" or "none" of a T7 genome entered the Escherichia coli cell after NUV treatment. Further studies with radioactively labeled phage indicated that irradiated phage failed to adsorb to host cells. This damage by NUV was compared with the protein-DNA cross-link observed previously, when phage particles were irradiated with NUV in the presence of H2O2. H2O2 (in nonlethal concentration) acts synergistically with NUV so that equivalent phage inactivation is achieved by much lower irradiation doses.
近紫外光（NUV）（300至400纳米）和远紫外光（FUV）（254纳米）辐射通过不同机制损伤噬菌体。在FUV照射后可观察到宿主细胞复活、韦格勒复活和多重复活，但在NUV照射后则未观察到。此外，在FUV照射后，鼠伤寒沙门氏菌中his +重组体的数量随P22噬菌体转导而增加，但在NUV照射后则不然。NUV照射后这种复活和重组的丧失不一定是由于宿主无法修复噬菌体损伤。相反，在NUV照射后，噬菌体基因组无法进入宿主细胞。对于NUV照射的T7噬菌体，这是通过与琥珀突变体的遗传杂交确定的，这表明在NUV处理后，T7基因组要么“全部”要么“没有一个”进入大肠杆菌细胞。用放射性标记噬菌体进行的进一步研究表明，受照射的噬菌体无法吸附到宿主细胞上。将这种NUV造成的损伤与之前观察到的蛋白质-DNA交联进行了比较，当时在过氧化氢存在的情况下用NUV照射噬菌体颗粒。过氧化氢（在非致死浓度下）与NUV协同作用，因此通过低得多的照射剂量就能实现等效的噬菌体灭活。
