Kerchove Alexis J de, Elimelech Menachem
Environmental Engineering Program, Department of Chemical Engineering, Yale University, P.O. Box 208286, New Haven, Connecticut 06520-8286, USA.
Langmuir. 2008 Apr 1;24(7):3392-9. doi: 10.1021/la7036229. Epub 2008 Feb 27.
We investigated the impact of calcium and magnesium ions on the deposition kinetics of flagellated and nonflagellated Pseudomonas aeruginosa onto an alginate conditioning film in a radial stagnation point flow system. The bacterial deposition/adhesion behavior was related to structural changes of the alginate film in the presence of the divalent cations. Our results showed that adhesion of nonmotile bacteria was governed by cation bridging interactions between high-affinity sites at the bacterial surface and either clean or alginate-conditioned substrate surfaces. For motile bacteria, the adhesion onto clean quartz was governed by electrostatic interactions while adhesion onto alginate-conditioned quartz was dependent on the structure and viscoelastic properties of the alginate film in the presence of calcium or magnesium. We demonstrate that bacterial adhesion behavior is governed both by the effects of divalent cations on the surface properties of the bacteria and the substrate and by the type of specific interactions occurring between these two surfaces.
我们在径向驻点流系统中研究了钙离子和镁离子对有鞭毛和无鞭毛的铜绿假单胞菌在藻酸盐调理膜上沉积动力学的影响。细菌的沉积/黏附行为与二价阳离子存在时藻酸盐膜的结构变化有关。我们的结果表明，非运动性细菌的黏附受细菌表面高亲和力位点与清洁或藻酸盐调理的底物表面之间的阳离子桥连相互作用控制。对于运动性细菌，在清洁石英上的黏附受静电相互作用控制，而在藻酸盐调理的石英上的黏附则取决于藻酸盐膜在钙或镁存在下的结构和粘弹性特性。我们证明，细菌的黏附行为既受二价阳离子对细菌和底物表面性质的影响，也受这两个表面之间发生的特定相互作用类型的控制。
