Goker H, Haznedaroglu I C, Ercetin S, Kirazli S, Akman U, Ozturk Y, Firat H C
Department of Haematology, Hacettepe University Medical School, Ankara, Turkey.
J Int Med Res. 2008 Jan-Feb;36(1):163-70. doi: 10.1177/147323000803600121.
Ankaferd Blood Stopper (ABS), a standardized mixture of five plants, has been used historically as a haemostatic agent but its mechanism of action remains unknown. This study investigated the in vitro effects of ABS on haemostatic parameters. When added to plasma or serum, ABS induced the very rapid formation of a protein network and erythrocyte aggregation. The levels of coagulation factors II, V, VII, VIII, IX, X, XI, and XIII were not affected by ABS. Plasma fibrinogen activity and antigen levels were decreased following the addition of ABS, in parallel with the prolonged thrombin time. Total protein, albumin, and globulin levels decreased after the addition of ABS. Our findings suggest that ABS stimulates the formation of an encapsulated protein network that provides focal points for erythrocyte aggregation. ABS has the therapeutic potential to be used for the management of haemorrhage and this agent should be investigated further in clinical trials.
安卡菲德止血剂(ABS)是一种由五种植物组成的标准化混合物,历史上一直被用作止血剂,但其作用机制尚不清楚。本研究调查了ABS对止血参数的体外影响。当添加到血浆或血清中时,ABS会诱导蛋白质网络的快速形成和红细胞聚集。凝血因子II、V、VII、VIII、IX、X、XI和XIII的水平不受ABS影响。添加ABS后,血浆纤维蛋白原活性和抗原水平降低,同时凝血酶时间延长。添加ABS后,总蛋白、白蛋白和球蛋白水平降低。我们的研究结果表明,ABS刺激形成一种被包裹的蛋白质网络,为红细胞聚集提供聚集点。ABS具有用于治疗出血的潜力,应在临床试验中进一步研究这种药物。