Sutherland J C, Emrick A B, Trunk J
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
Electrophoresis. 1989 May-Jun;10(5-6):315-7. doi: 10.1002/elps.1150100508.
The maximum length of DNA molecules that can be separated by gel electrophoresis can be increased greatly by periodically altering the direction of the electric field with respect to the gel by an angle that exceeds 90 degrees. One method involves rotating the gel by the desired angle in alternate directions periodically during electrophoresis. We describe a modification of the rotating gel electrophoresis apparatus developed by Serwer (Electrophoresis 1987, 8, 301-304) that uses a pneumatic rotary actuator instead of a stepping motor, hence reducing the cost by about 50%. Other advantages of our design are a lower center of gravity that makes the apparatus more stable and the removal of all electrical power from beneath the fluid-filled electrophoresis chamber. We present data demonstrating the separation of chromosomal length DNA molecules from Saccharomyces cerevisiae strain 334 into 14 resolved bands in parallel lanes.
通过以超过90度的角度周期性地改变电场相对于凝胶的方向，可以大大增加通过凝胶电泳分离的DNA分子的最大长度。一种方法是在电泳过程中以交替方向将凝胶按所需角度周期性旋转。我们描述了对Serwer开发的旋转凝胶电泳装置（《电泳》，1987年，第8卷，301 - 304页）的一种改进，该改进使用气动旋转执行器而非步进电机，从而使成本降低约50%。我们设计的其他优点包括重心较低，使装置更稳定，以及去除了充满液体的电泳槽下方的所有电力。我们展示的数据表明，从酿酒酵母菌株334中分离出的染色体长度的DNA分子在平行泳道中被分离成14条清晰的条带。
