Herrero Miguel, Cacciola Francesco, Donato Paola, Giuffrida Daniele, Dugo Giacomo, Dugo Paola, Mondello Luigi
Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, Viale Annunziata, 98168 Messina, Italy.
J Chromatogr A. 2008 Apr 25;1188(2):208-15. doi: 10.1016/j.chroma.2008.02.039. Epub 2008 Feb 15.
High-performance liquid chromatography is considered as the selected analytical tool for a huge number of applications, including the carotenoid analysis. However, due to the great complexity of some natural samples containing this kind of compounds, conventional LC could not have enough separation power. In this work, serial connection of several columns is proposed as an alternative to LC and also to comprehensive two-dimensional LC for the analysis of carotenoids from complex real matrices. Different parameters regarding these separation procedures are studied and discussed, such as the stationary phase used or the application of high separation temperatures. The applicability of connecting two C30 columns to significantly increase the separation power, resolution and peak capacity for the analysis of carotenoids has been demonstrated for the first time. In fact, a peak capacity of 79 was obtained when using two C30 serial coupled columns, compared to 61 achieved using a single column. Similar improvements were also observed for the other serial couplings studied. The present methodology could be applied to the analysis of carotenoids in a great variety of samples. To the best of our knowledge, this is the first development carried out to study natural products such as carotenoids using columns coupled in series.
高效液相色谱法被认为是适用于大量应用的首选分析工具，包括类胡萝卜素分析。然而，由于某些含有此类化合物的天然样品极为复杂，传统液相色谱法的分离能力不足。在这项工作中，提出串联几根色谱柱，作为液相色谱法以及全二维液相色谱法的替代方法，用于分析复杂实际基质中的类胡萝卜素。研究并讨论了与这些分离程序相关的不同参数，例如所使用的固定相或高分离温度的应用。首次证明了连接两根C30色谱柱可显著提高类胡萝卜素分析的分离能力、分辨率和峰容量。事实上，使用两根串联的C30色谱柱时获得了79的峰容量，而使用单根色谱柱时为61。在所研究的其他串联组合中也观察到了类似的改进。本方法可应用于多种样品中类胡萝卜素的分析。据我们所知，这是首次开展的使用串联色谱柱研究类胡萝卜素等天然产物的工作。
