University of Hawai'i Cancer Center, Honolulu, HI 96813, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jul 15;931:23-41. doi: 10.1016/j.jchromb.2013.04.031. Epub 2013 May 9.
Retinol, tocopherols, coenzyme Q10, carotenoids, and vitamin D are lipophilic compounds shown to function as important health-protective agents by mitigating the damaging effects of oxidative and other injury. Scientific interest in evaluating these compounds has resurfaced in recent years, particularly in the nutritional, clinical and epidemiologic fields, and has precipitated the development of a multitude of new analytical techniques. This review considers recent developments in HPLC-based assays since 2007 for the simultaneous determination of these lipid-phase compounds utilizing exclusively serum or plasma as these matrices are mostly used in clinical and epidemiological investigations. We also provide an overview of blood measurements for selected carotenoids, tocopherols, coenzyme Q10 and retinol from the last 15years of healthy umbilical cord blood, children, and adults.
视黄醇、生育酚、辅酶 Q10、类胡萝卜素和维生素 D 是亲脂性化合物，通过减轻氧化和其他损伤的破坏性影响，被证明是重要的健康保护剂。近年来，人们对评估这些化合物的科学兴趣重新出现，特别是在营养、临床和流行病学领域，并促成了大量新分析技术的发展。本综述考虑了 2007 年以来基于 HPLC 的分析方法的最新进展，这些方法仅利用血清或血浆作为这些基质来同时测定这些脂质相化合物，因为这些基质在临床和流行病学研究中大多被使用。我们还概述了过去 15 年中取自健康脐带血、儿童和成人的选定类胡萝卜素、生育酚、辅酶 Q10 和视黄醇的血液测量值。
