Bruzzoniti Maria Concetta, De Carlo Rosa Maria, Horvath Krisztian, Perrachon Daniela, Prelle Ambra, Tófalvi Renáta, Sarzanini Corrado, Hajós Péter
Department of Analytical Chemistry, University of Torino, Via P. Giuria 5, 10125 Torino, Italy.
J Chromatogr A. 2008 Apr 11;1187(1-2):188-96. doi: 10.1016/j.chroma.2008.02.028. Epub 2008 Feb 13.
A new high performance ion chromatographic method has been developed for the separation of the nine chlorinated-brominated haloacetic acids (HAAs) that are the disinfection by-products of chlorination of drinking water, using a macrocycle-based adjustable-capacity anion-exchange separator column (IonPac Cryptand A1). A gradient method based on theoretical and experimental considerations has been optimized in which 10 mM NaOH-LiOH step gradient was performed at the third minute of the analysis. The optimized method allowed us to separate the nine HAAs and seven possibly interfering inorganic anions in less than 25 min with acceptable resolution. The minimum concentrations detectable for HAAs were between 8.0 (MBA) and 210 (TBA) microg L(-1), with linearity included between 0.9947 (TBA) and 0.9998 (MBA). To increase sensitivity, a 25-fold preconcentration step on a reversed phase substrate (LiChrolut EN) has been coupled. Application of this method to the analysis of haloacetic acids in real tap water samples is illustrated.
已开发出一种新型高效离子色谱方法，用于分离九种氯代 - 溴代卤乙酸（HAAs），这些卤乙酸是饮用水氯化消毒的副产物，采用基于大环的可调容量阴离子交换分离柱（IonPac Cryptand A1）。基于理论和实验考虑，优化了一种梯度方法，即在分析的第三分钟进行10 mM NaOH - LiOH阶梯梯度。优化后的方法使我们能够在不到25分钟的时间内分离出九种HAAs和七种可能干扰的无机阴离子，分辨率可接受。HAAs的最低检测浓度在8.0（一溴乙酸）至210（三溴乙酸）μg L⁻¹之间，线性范围在0.9947（三溴乙酸）至0.9998（一溴乙酸）之间。为提高灵敏度，已结合在反相基质（LiChrolut EN）上进行25倍预浓缩步骤。文中展示了该方法在实际自来水样品中卤乙酸分析的应用。
