Chaves J Q, Cavados C F G, Rabinovitch L
Laboratório de Fisiologia Bacteriana, Instituto Oswaldo Cruz-FIOCRUZ, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil.
J Invertebr Pathol. 2008 May;98(1):85-92. doi: 10.1016/j.jip.2008.01.002. Epub 2008 Jan 16.
A total of 28 autoagglutinating strains of Bacillus thuringiensis were isolated from different ecologic niches and distinct sites. Twenty-six strains demonstrated toxicity to mosquito larvae of Aedes aegypti and Culex quinquefasciatus. The electrophoretic protein profiles of the crystal components were studied. Twenty-three out of the 28 strains showed the same larvicidal activity and the same protein profiles as B. thuringiensis serovar israelensis. Using isoenzyme analysis (MLEE), it was observed the presence of three electrophoretic types (ETs). The mosquitocidal strains grouped into one ET. The random amplified polymorphic DNA analysis (RAPD) was evaluated using six primers, which demonstrated three different patterns for the 28 autoagglutinating strains, allowing correlation of the profiles obtained with the toxicity observed in the bioassays. The RAPD patterns for mosquitocidal strains were identical to the one of serovar israelensis. However, to strains of low toxicity, each primer generated distinctive RAPD patterns, which demonstrated that these strains belong to different serovars. Although the antigenic classification the 26 autoagglutinating strains of B. thuringiensis could not be determined by classical flagellar serotyping, MLEE and RAPD profiles proved these strains to be compatible with B. thuringiensis serovar israelensis.
从不同生态位和不同地点共分离出28株自凝性苏云金芽孢杆菌菌株。26株菌株对埃及伊蚊和致倦库蚊幼虫表现出毒性。研究了晶体成分的电泳蛋白质谱。28株菌株中有23株表现出与苏云金芽孢杆菌以色列血清型相同的杀幼虫活性和相同的蛋白质谱。通过同工酶分析（MLEE），观察到存在三种电泳类型（ETs）。杀蚊菌株归为一个ET。使用六种引物评估随机扩增多态性DNA分析（RAPD），结果显示28株自凝性菌株呈现出三种不同模式，从而可以将所得图谱与生物测定中观察到的毒性相关联。杀蚊菌株的RAPD模式与以色列血清型的相同。然而，对于低毒性菌株，每种引物产生独特的RAPD模式，这表明这些菌株属于不同的血清型。尽管无法通过经典鞭毛血清分型确定26株自凝性苏云金芽孢杆菌菌株的抗原分类，但MLEE和RAPD图谱证明这些菌株与苏云金芽孢杆菌以色列血清型相符。
