利用随机扩增多态性DNA（RAPD）技术对苏云金芽孢杆菌某些血清型的遗传多样性进行表征

Characterization of genetic diversity of some serovars of Bacillus thuringiensis by RAPD.

作者信息

Pattanayak D, Chakrabarti S K, Kumar P A, Naik P S

机构信息

Molecular Biology Laboratory, Division of Crop Improvement, Central Potato Research Institute, Shimla, India.

出版信息

Indian J Exp Biol. 2001 Sep;39(9):897-901.

PMID:11831372

Abstract

RAPD based fingerprinting of 21 serovars of Bacillus thuringiensis (Bt) representing different serotypes was performed using 19 random decamer primers. A total of 172 polymorphic fragments, ranging in size from 161-2789 bp, were amplified from 13 of the 19 primers. Pairwise genetic similarity analysis revealed very low similarity values, ranging from 3-68%, among the serovars of Bt, indicating high genetic divergence. Nineteen serovars of Bt fell in two major clusters and remaining two formed solitary clusters in the dendogram. Clustering of Bt strains established genetic relatedness between serovars and serotypes. It has been suggested that RAPD analysis can be used for genotypic characterization of Bt to complement flagellar serotyping.

摘要

使用19个随机十聚体引物对代表不同血清型的21个苏云金芽孢杆菌（Bt）血清型进行了基于RAPD的指纹图谱分析。从19个引物中的13个引物共扩增出172个多态性片段，大小在161 - 2789 bp之间。两两遗传相似性分析显示，Bt血清型之间的相似性值非常低，范围为3% - 68%，表明遗传差异很大。19个Bt血清型在树状图中分为两个主要聚类，其余两个形成单独的聚类。Bt菌株的聚类建立了血清型和血清型之间的遗传相关性。有人提出，RAPD分析可用于Bt的基因型表征，以补充鞭毛血清分型。