Bugden J B C, Yeung C W, Kepkay P E, Lee K
Centre for Offshore Oil and Gas Environmental Research, Fisheries and Oceans Canada, Bedford Institute of Oceanography, 1 Challenger Drive, P.O. Box 1006, Dartmouth, NS, Canada B2Y 4A2.
Mar Pollut Bull. 2008 Apr;56(4):677-85. doi: 10.1016/j.marpolbul.2007.12.022. Epub 2008 Mar 4.
Excitation-emission matrix spectroscopy (EEMS) was used to characterize the ultra violet fluorescence fingerprints of eight crude oils (with a 14,470-fold range of dynamic viscosity) in seawater. When the chemical dispersant Corexit 9500 was mixed with the oils prior to their dispersion in seawater, the fingerprints of each oil changed primarily as an increase in fluorescence over an emission band centered on 445 nm. In order to simplify the wealth of information available in the excitation-emission matrix spectra (EEMs), two ratios were calculated. A 66-90% decrease in the slope ratio was observed with the addition of Corexit. When the slope ratios were reduced in complexity to intensity ratios, similar trends were apparent. As a result either of the ratios could be used as a simple and rapid means of identifying and monitoring chemically dispersed oil in the open ocean.
采用激发-发射矩阵光谱法（EEMS）对海水中8种原油（动态粘度范围为14470倍）的紫外荧光指纹图谱进行了表征。当在原油分散于海水之前将化学分散剂Corexit 9500与原油混合时，每种原油的指纹图谱主要发生变化，即荧光在以445 nm为中心的发射带上增加。为了简化激发-发射矩阵光谱（EEMs）中可用的大量信息，计算了两个比率。添加Corexit后，斜率比下降了66-90%。当将斜率比简化为强度比时，类似的趋势也很明显。因此，这两个比率中的任何一个都可以用作识别和监测公海中化学分散油的简单快速方法。
