Weidner U, Sackmann U, Nehls U, Weiss H
Institut für Biochemie, Universität Düsseldorf, F.R.G.
Biochim Biophys Acta. 1991 Jul 23;1089(3):391-2. doi: 10.1016/0167-4781(91)90182-l.
The primary structure of the nuclear-encoded 18.3 kDa subunit of the respiratory chain NADH: ubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA and the N-terminus of the protein. The cDNA contains an open reading frame for a protein of 206 amino acids. The mature protein consists of 173 amino acids and has a molar mass of 18,341 Da. The precursor protein includes a characteristic mitochondrial import sequence with a typical matrix peptidase processing site.
通过对cDNA和该蛋白的N端进行测序,确定了粗糙脉孢菌呼吸链NADH:泛醌还原酶(复合体I)的核编码18.3 kDa亚基的一级结构。该cDNA包含一个编码206个氨基酸的蛋白质的开放阅读框。成熟蛋白由173个氨基酸组成,摩尔质量为18,341 Da。前体蛋白包括一个具有典型基质肽酶加工位点的特征性线粒体导入序列。