粗糙脉孢菌复合体I 29/21-kDa亚基的一级结构、体外表达及线粒体导入
Primary structure, in vitro expression and import into mitochondria of a 29/21-kDa subunit of complex I from Neurospora crassa.
作者信息
Videira A, Tropschug M, Werner S
机构信息
Institut für Physiologische Chemie der Universität München, Federal Republic of Germany.
出版信息
Biochem Biophys Res Commun. 1990 Jan 15;166(1):280-5. doi: 10.1016/0006-291x(90)91942-l.
A full-length cDNA clone coding for a cytoplasmically-synthesized subunit of complex I from Neurospora crassa (apparent molecular mass of 29 kDa) was isolated. DNA sequencing revealed an open reading frame coding for a protein containing 201 amino acids. A molecular mass of 21323 Da was calculated. The precursor polypeptide was efficiently expressed in vitro and imported into isolated mitochondria. It is synthesized without a cleavable signal sequence and needs a membrane potential in order to bind to the mitochondrial membranes.
分离出了一个编码来自粗糙脉孢菌复合体I胞质合成亚基（表观分子量为29 kDa）的全长cDNA克隆。DNA测序揭示了一个编码含201个氨基酸蛋白质的开放阅读框。计算出分子量为21323 Da。前体多肽在体外高效表达并导入分离的线粒体中。它在合成时没有可裂解的信号序列，并且需要膜电位才能与线粒体膜结合。
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