Hemström Petrus, Nygren Yvonne, Björn Erik, Irgum Knut
Department of Chemistry, Umeå University, Umeå, Sweden.
J Sep Sci. 2008 Mar;31(4):599-603. doi: 10.1002/jssc.200700480.
Several low volatile organic solvents were evaluated as organic modifiers in eluents for HILIC separations of cisplatin species to optimize the on-line coupling of HILIC to inductively coupled plasma MS (ICP-MS). The aim was to identify a solvent giving low solvent vapor loading of the ICP, to maximize analyte sensitivity and minimize carbon depositions on instrumental parts, while retaining chromatographic performance. The best overall performance of the HILIC-ICP-MS system for the analysis of cisplatin was achieved using 1,4-dioxane as eluent, yielding high retention and an HILIC type retention mechanism, at the expense of a 50% drop in column efficiency due to the higher viscosity of 1,4-dioxane compared to the more commonly used HILIC solvent ACN. Using 1,4-dioxane as solvent in HILIC provides the best compromise between carbon deposition and separation efficiency among a series of high-boiling water-miscible solvents tested.
评估了几种低挥发性有机溶剂作为亲水作用色谱（HILIC）分离顺铂物种洗脱液中的有机改性剂，以优化HILIC与电感耦合等离子体质谱（ICP-MS）的在线联用。目的是确定一种能使ICP的溶剂蒸汽负载量低、最大化分析物灵敏度并最小化仪器部件上碳沉积的溶剂，同时保持色谱性能。使用1,4-二氧六环作为洗脱液，HILIC-ICP-MS系统在分析顺铂时实现了最佳的整体性能，产生了高保留率和HILIC型保留机制，但由于1,4-二氧六环的粘度高于更常用的HILIC溶剂乙腈（ACN），柱效下降了50%。在一系列测试的高沸点与水混溶的溶剂中，在HILIC中使用1,4-二氧六环作为溶剂能在碳沉积和分离效率之间提供最佳折衷。
