Nguyen Hien P, Schug Kevin A
Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, USA.
J Sep Sci. 2008 May;31(9):1465-80. doi: 10.1002/jssc.200700630.
The analysis of highly hydrophilic, ionic, and polar compounds has been performed by HILIC-ESI-MS for the last few years. The use of low aqueous/high polar organic solvent content in HILIC separation mobile phase is almost ideal for ESI-MS detection in many cases, resulting in increased sensitivity. Although the addition of modifiers such as acids or salts is necessary in some circumstances for a good separation, the optimum concentrations used are still highly amenable for ESI-MS analysis, showing few deleterious effects. In this review, the mechanism of HILIC separation and ESI ion generation will be briefly discussed, followed by a summary of method development and applications in several fields of research including pharmaceutical, biomolecular, food, metabolic, and environmental analysis.
在过去几年中，亲水作用色谱-电喷雾电离质谱（HILIC-ESI-MS）已用于对高亲水性、离子型和极性化合物进行分析。在亲水作用色谱分离流动相中使用低水相/高极性有机溶剂含量，在许多情况下对于电喷雾电离质谱检测几乎是理想的，可提高灵敏度。尽管在某些情况下为实现良好分离需要添加酸或盐等改性剂，但所使用的最佳浓度对于电喷雾电离质谱分析仍然非常适用，几乎没有有害影响。在本综述中，将简要讨论亲水作用色谱分离和电喷雾电离离子产生的机制，随后总结方法开发及其在药物、生物分子、食品、代谢和环境分析等几个研究领域中的应用。
