利用单克隆抗体对猫源和牛源鹦鹉热衣原体进行抗原分析。
Antigenic analysis of feline and bovine Chlamydia psittaci with monoclonal antibodies.
作者信息
Matsuno H, Fukushi H, Yamaguchi T, Hirai K
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
出版信息
J Vet Med Sci. 1991 Apr;53(2):173-9. doi: 10.1292/jvms.53.173.
Monoclonal antibodies were established for antigenic analysis of feline and bovine Chlamydia psittaci. The monoclonal antibodies recognized lipopolysaccharide (LPS), 56-64, 84 or 86 kDa antigens. At least 5 antibody-binding sites were detected on LPS with the monoclonal antibodies. The 56-64 kDa antigen was suggested to have both polypeptide and carbohydrate antibody binding sites. Immunoblotting analysis of cat and cattle sera indicated that the 56-64 kDa antigen is an important antigen in host immune response. The monoclonal antibodies are extremely useful tools to analyse the structure and function of chlamydial antigens.
建立了用于猫和牛鹦鹉热衣原体抗原分析的单克隆抗体。这些单克隆抗体识别脂多糖（LPS）、56 - 64 kDa、84 kDa或86 kDa抗原。用单克隆抗体在LPS上检测到至少5个抗体结合位点。推测56 - 64 kDa抗原同时具有多肽和碳水化合物抗体结合位点。猫和牛血清的免疫印迹分析表明，56 - 64 kDa抗原是宿主免疫反应中的一种重要抗原。这些单克隆抗体是分析衣原体抗原结构和功能的极其有用的工具。
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