Vanrompay D, Andersen A A, Ducatelle R, Haesebrouck F
Department of Avian Diseases, Faculty of Veterinary Medicine, State University of Ghent, Belgium.
J Clin Microbiol. 1993 Jan;31(1):134-7. doi: 10.1128/jcm.31.1.134-137.1993.
A panel of five serovar-specific monoclonal antibodies which distinguish the five known avian serovars of Chlamydia psittaci was used to serotype 45 European avian Chlamydia psittaci isolates. Chlamydial antigen was grown in Buffalo green monkey (BGM) cells or in embryonated chicken eggs and was then inoculated into BGM cells. Serotyping was performed in an indirect immunofluorescence test. The 45 European isolates included 22 isolates from the order Psittaciformes, 9 isolates from the order Columbiformes, 6 isolates from the order Galliformes, 5 isolates from the order Passeriformes, and 3 isolates from the order Anseriformes. All of these were successfully serotyped. No additional serovars were found. One isolate from a duck and two isolates from psittacine birds gave positive immunofluorescences with two monoclonal antibodies considered to be specific for two different serovars. These three isolates were cloned by an agar overlay method. Serotyping of the clones demonstrated that the duck and one psittacine bird each were infected with two different serovars. After cloning, one isolate from a psittacine bird reacted only with serovar A. From these results it was concluded that this serotyping system allows the classification of all isolates tested so far. The results show that similar serovars are prevalent in avian species in Europe and the United States. The results also indicate that birds from a certain order are more susceptible to a distinct serovar. The use of a panel of serovar-specific monoclonal antibodies in the immunofluorescence test provides a reliable method for serotyping avian isolates. Monoclonal antibodies to new avian isolate serovars can easily be added to the panel, which makes the system useful for epidemiological studies.
使用一组可区分鹦鹉热衣原体已知的五种禽血清型的血清型特异性单克隆抗体，对45株欧洲禽鹦鹉热衣原体分离株进行血清分型。衣原体抗原在布法罗绿猴（BGM）细胞或鸡胚中培养，然后接种到BGM细胞中。血清分型在间接免疫荧光试验中进行。这45株欧洲分离株包括来自鹦形目的22株、鸽形目的9株、鸡形目的6株、雀形目的5株和雁形目的3株。所有这些分离株均成功进行了血清分型，未发现其他血清型。一只鸭的分离株和两只鹦鹉类鸟的分离株与被认为对两种不同血清型具有特异性的两种单克隆抗体产生了阳性免疫荧光。这三株分离株通过琼脂覆盖法进行克隆。克隆株的血清分型表明，鸭和一只鹦鹉类鸟各自感染了两种不同的血清型。克隆后，一株鹦鹉类鸟的分离株仅与血清型A反应。从这些结果得出结论，该血清分型系统能够对迄今为止测试的所有分离株进行分类。结果表明，欧洲和美国的禽类物种中普遍存在相似的血清型。结果还表明，某一目的鸟类更容易感染某一特定血清型。在免疫荧光试验中使用一组血清型特异性单克隆抗体为禽类分离株的血清分型提供了一种可靠的方法。针对新的禽类分离株血清型的单克隆抗体可以很容易地添加到该组中，这使得该系统对流行病学研究有用。
