Department of Obstetrics and Gynecology, IVF Unit, The Chaim Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel; affiliated to Sackler School of Medicine, Tel Aviv University , Tel Aviv , Israel.
Gynecol Endocrinol. 2014 Mar;30(3):202-4. doi: 10.3109/09513590.2013.875995. Epub 2014 Jan 7.
To compare the outcome of vitrification versus slow freezing cryopreservation for cleavage stage day 2-3 embryos.
A retrospective observational study.
All thawed embryos assisted reproduction cycles between January 2010 and December 2012 at a single IVF laboratory of a Tertiary Medical Center.
Five hundred and thirty-nine cycles of day 2-3 thawed embryos.
In 327 of the thawed cycles, the embryos were vitrified and in 212 of the cycles the embryos were derived from slow freezing embryos.
Embryo survival rate, blastomere surviving rate and pregnancy rate.
Embryo survival rate was significantly higher after vitrification compared with slow freezing (81.6%, 647/793 versus 70.0%, 393/562 embryos, p < 0.0001). The clinical pregnancy rate per ET was significantly higher following vitrification compared to slow freezing, 20.0%, 63/314 versus 11.9%, 23/193, respectively (p = 0.02).
Vitrification of day 2-3 cleavage stage embryos yields better cycle outcome in all the parameters compared to slow freezing.
比较玻璃化与慢速冷冻对卵裂期第 2-3 天胚胎的冷冻效果。
回顾性观察研究。
单家三级医疗中心的一个 IVF 实验室，所有解冻胚胎的辅助生殖周期都在 2010 年 1 月至 2012 年 12 月间进行。
539 个第 2-3 天解冻胚胎周期。
在 327 个解冻周期中，胚胎进行玻璃化冷冻，在 212 个周期中，胚胎来自慢速冷冻胚胎。
胚胎存活率、卵裂球存活率和妊娠率。
与慢速冷冻相比，玻璃化冷冻后的胚胎存活率显著更高（81.6%，647/793 个胚胎比 70.0%，393/562 个胚胎，p<0.0001）。玻璃化冷冻后每个胚胎移植的临床妊娠率显著高于慢速冷冻，分别为 20.0%，63/314 个胚胎比 11.9%，23/193 个胚胎（p=0.02）。
与慢速冷冻相比，玻璃化冷冻第 2-3 天卵裂期胚胎在所有参数上均能获得更好的周期结果。
