Lajunen Taina, Vikatmaa Pirkka, Ikonen Tuija, Lepäntalo Mauri, Lounatmaa Kari, Sormunen Raija, Rantala Aino, Leinonen Maija, Saikku Pekka
Respiratory Infection Unit, National Public Health Institute, Oulu, Finland.
Diagn Microbiol Infect Dis. 2008 Jun;61(2):156-64. doi: 10.1016/j.diagmicrobio.2008.01.006. Epub 2008 Mar 4.
Chlamydia pneumoniae has been associated with cardiovascular diseases and has been shown by different methods to be present in atherosclerotic lesions. However, not all studies have found C. pneumoniae in atherosclerotic tissues. We compared polymerase chain reaction (PCR) methods, in situ hybridization (ISH), and measurement of chlamydial lipopolysaccharide (cLPS) by enzyme immunoassay (EIA) from homogenized atherosclerotic tissue in the detection of C. pneumoniae. In a study population of 110 patients with carotid artery disease, cLPS was found in 22.2%, and DNA by PCR was found in 34.3% and by ISH in 39.4% of the samples. Poor repeatability was shown to complicate PCR, and the technical problems inherent in ISH were not insignificant. In contrast, the cLPS EIA test was fast and easy to perform. If the sensitivity could be increased, for example, by testing multiple tissue pieces, cLPS EIA might provide a standardized commercial method for the detection of chlamydia in tissue samples, and it, thus, merits further characterization and validation in different patient populations.
肺炎衣原体与心血管疾病有关，并且已通过不同方法证明其存在于动脉粥样硬化病变中。然而，并非所有研究都在动脉粥样硬化组织中发现肺炎衣原体。我们比较了聚合酶链反应（PCR）方法、原位杂交（ISH）以及通过酶免疫测定（EIA）对匀浆化动脉粥样硬化组织中衣原体脂多糖（cLPS）的测量，以检测肺炎衣原体。在110名颈动脉疾病患者的研究人群中，22.2%的样本检测到cLPS，34.3%的样本通过PCR检测到DNA，39.4%的样本通过ISH检测到DNA。结果显示PCR的重复性较差，ISH固有的技术问题也不容忽视。相比之下，cLPS EIA检测快速且易于操作。如果通过检测多个组织块等方式提高其灵敏度，cLPS EIA可能为组织样本中衣原体的检测提供一种标准化的商业方法，因此，它值得在不同患者群体中进一步表征和验证。
