用于蛋白质标记的氯化荧光素的合成。

Synthesis of chlorinated fluoresceins for labeling proteins.

作者信息

Tian Min, Wu Xiang-Long, Zhang Bin, Li Jian-Li, Shi Zhen

机构信息

Department of Chemistry, Northwest University, Xi'an 710069, China.

出版信息

Bioorg Med Chem Lett. 2008 Mar 15;18(6):1977-9. doi: 10.1016/j.bmcl.2008.01.114. Epub 2008 Feb 2.

DOI:10.1016/j.bmcl.2008.01.114

PMID:18308564

Abstract

Two novel chlorinated fluoresceins 4,7,2',7'-tetrachloro-6-(5-carboxypentyl)fluorescein (8a) and 4,7,4',5'-tetra-chloro-6-(5-carboxypentyl)fluorescein (8b) were synthesized as fluorescent probes for labeling proteins. These two fluoresceins contain 6-aminohexanoic acid as spacer linker to minimize the fluorescence quenching of the fluorescein molecules by the proteins to be labeled.

摘要

合成了两种新型氯化荧光素4,7,2',7'-四氯-6-(5-羧基戊基)荧光素(8a)和4,7,4',5'-四氯-6-(5-羧基戊基)荧光素(8b)作为蛋白质标记的荧光探针。这两种荧光素含有6-氨基己酸作为间隔连接体，以尽量减少待标记蛋白质对荧光素分子荧光猝灭的影响。