两种新型荧光蛋白标记探针的合成及生物学应用
Synthesis and biological applications of two novel fluorescent proteins-labeling probes.
作者信息
Wu Xiang-Long, Tian Min, He Huai-Zhen, Sun Wei, Li Jian-Li, Shi Zhen
机构信息
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi' an, China.
出版信息
Bioorg Med Chem Lett. 2009 Jun 1;19(11):2957-9. doi: 10.1016/j.bmcl.2009.04.049. Epub 2009 Apr 18.
Two novel chlorinated fluoresceins 2',4',5',7'-tetrachloro-6-(5-carboxypentyl)-4,7-dichloro fluorescein succinimidyl ester (1G) and 2',4',5',7'-tetrachloro-6-(3-carboxypropyl)-4,7-dichlorofluorescein succinimidyl ester (2G) were synthesized as fluorescent probes for labeling proteins. Structures of target compounds and intermediates were determined via IR, MS, (1)H NMR and element analysis. The investigation in immunofluorescence histochemistry showed them had strong fluorescence, high photostability and good biocompatibility.
合成了两种新型氯化荧光素2',4',5',7'-四氯-6-(5-羧基戊基)-4,7-二氯荧光素琥珀酰亚胺酯(1G)和2',4',5',7'-四氯-6-(3-羧基丙基)-4,7-二氯荧光素琥珀酰亚胺酯(2G)作为蛋白质标记荧光探针。通过红外光谱、质谱、核磁共振氢谱和元素分析确定了目标化合物及中间体的结构。免疫荧光组织化学研究表明它们具有强荧光、高光稳定性和良好的生物相容性。
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