Van Kaer L, Wu M, Ichikawa Y, Ito K, Bonneville M, Ostrand-Rosenberg S, Murphy D B, Tonegawa S
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
Immunol Rev. 1991 Apr;120:89-115. doi: 10.1111/j.1600-065x.1991.tb00589.x.
We have studied the ligand specificity of a gamma delta T-cell receptor (TCR) derived from a mouse T-cell hybridoma (KN6). KN6 cells reacted with syngeneic (C57BL/6) cells from various origins (splenocytes, thymocytes, peritoneal exudate cells, etc.) and cells from many different mouse strains. KN6 reactivity against cells from a panel of congenic and recombinant mouse strains demonstrated that the ligand recognized by KN6 is controlled by an MHC-linked gene that most probably maps in the TL region. We cloned this gene and formally proved that it does map in the TL region. This gene turned out to be a novel class I gene (designated T22b) belonging to a hitherto unidentified cluster of TL region genes in strain C57BL/6. This gene was expressed in many different tissues and cell types. We also examined the tissue expression of several other TL genes. One of these, the structural gene (T3b) encoding the thymus leukemia (TL) antigen from C57BL/6 mice, was specifically expressed in the epithelium of the small intestine. Since the intestinal epithelium of the mouse is known to be the homing site for a subset of gamma delta T cells (i-IEL) bearing diverse TCR with V7 rearranged gamma chains, we propose that the T3b gene product is part of the ligand recognized by some of the i-IEL. Our data support the idea that gamma delta T cells might be specific for non-classical class I or class I-like molecules and suggest that gamma delta TCR and non-classical MHC co-evolved for the recognition of a conserved set of endogenous or foreign peptides.
我们研究了源自小鼠T细胞杂交瘤（KN6）的γδ T细胞受体（TCR）的配体特异性。KN6细胞可与来自各种来源的同基因（C57BL/6）细胞（脾细胞、胸腺细胞、腹腔渗出细胞等）以及来自许多不同小鼠品系的细胞发生反应。KN6对一组同源和重组小鼠品系细胞的反应性表明，KN6识别的配体受一个与MHC连锁的基因控制，该基因很可能定位于TL区域。我们克隆了这个基因，并正式证明它确实定位于TL区域。结果发现这个基因是一个新的I类基因（命名为T22b），属于C57BL/6品系中迄今未鉴定的TL区域基因簇。该基因在许多不同的组织和细胞类型中表达。我们还检测了其他几个TL基因的组织表达情况。其中之一，即编码C57BL/6小鼠胸腺白血病（TL）抗原的结构基因（T3b），在小肠上皮中特异性表达。由于已知小鼠的肠上皮是带有重排V7γ链的多种TCR的γδ T细胞亚群（i-IEL）的归巢部位，我们提出T3b基因产物是一些i-IEL识别的配体的一部分。我们的数据支持γδ T细胞可能对非经典I类或I类样分子具有特异性的观点，并表明γδ TCR和非经典MHC共同进化以识别一组保守的内源性或外源性肽。
