Li Jian-Rong, Wu Lan-Sheng, Zhang Guang-Ping
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(21):2277-81.
To investigate the improvement effects of Jincao tablet on immune function of the model of hysteromyoma in rat and the relationship between the model and pathogenesis.
Rats were randomly divided into 6 groups: normal group, model group, treatment groups including low,middle and high dosage groups of Jincao tablet and Guizhi Fuling pill. Rats were injected respectively with diethyl stilbestrol and progesterone. The immune apparatus of rats were measured. The levels of CD3, CD4 and CD4/ CD8 in serum were determined by flow cytometer. The estrogen and receptor were measured by radioligand binding assay and pathologic changes of womb tissue were observed microscopically.
Compared with normal group, the weight of thymus, the levels of CD3, CD4 and CD4/CD8 of model group were significantly decreased, and the levels of estrogen, estrogen receptor and CD8 were obviously increased. Jincao tablet groups were significant difference compared with model group and could alleviate the pathological changes of womb tissue.
Jincao tablet could improve the levels of immune function of the model of hysteromyoma in rat, and it might play a role in the pathogenesis of leiomyoma.
探讨金草片对大鼠子宫肌瘤模型免疫功能的改善作用以及该模型与发病机制之间的关系。
将大鼠随机分为6组：正常组、模型组、治疗组（包括金草片低、中、高剂量组及桂枝茯苓丸组）。分别给大鼠注射己烯雌酚和黄体酮。测量大鼠的免疫器官。采用流式细胞仪检测血清中CD3、CD4及CD4/CD8水平。采用放射性配体结合分析法检测雌激素及受体，并显微镜观察子宫组织的病理变化。
与正常组相比，模型组胸腺重量、CD3、CD4及CD4/CD8水平显著降低，雌激素、雌激素受体及CD8水平明显升高。金草片各治疗组与模型组相比有显著差异，且能减轻子宫组织的病理变化。
金草片可提高大鼠子宫肌瘤模型的免疫功能水平，可能在子宫肌瘤发病机制中发挥作用。
