Sapoznikov Anita, Pewzner-Jung Yael, Kalchenko Vyacheslav, Krauthgamer Rita, Shachar Idit, Jung Steffen
Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel.
Nat Immunol. 2008 Apr;9(4):388-95. doi: 10.1038/ni1571. Epub 2008 Mar 2.
Beyond its established function in hematopoiesis, the bone marrow hosts mature lymphocytes and acts as a secondary lymphoid organ in the initiation of T cell and B cell responses. Here we report the characterization of bone marrow-resident dendritic cells (bmDCs). Multiphoton imaging showed that bmDCs were organized into perivascular clusters that enveloped blood vessels and were seeded with mature B lymphocytes and T lymphocytes. Conditional ablation of bmDCs in these bone marrow immune niches led to the specific loss of mature B cells, a phenotype that could be reversed by overexpression of the antiapoptotic factor Bcl-2 in B cells. The presence of bmDCs promoted the survival of recirculating B cells in the bone marrow through the production of macrophage migration-inhibitory factor. Thus, bmDCs are critical for the maintenance of recirculating B cells in the bone marrow.
除了在造血过程中已确立的功能外，骨髓还容纳成熟淋巴细胞，并在启动T细胞和B细胞反应时充当次级淋巴器官。在此，我们报告了骨髓驻留树突状细胞（bmDCs）的特征。多光子成像显示，bmDCs组织成围绕血管的血管周围簇，并接种有成熟的B淋巴细胞和T淋巴细胞。在这些骨髓免疫龛中条件性消融bmDCs导致成熟B细胞特异性缺失，该表型可通过在B细胞中过表达抗凋亡因子Bcl-2来逆转。bmDCs的存在通过产生巨噬细胞迁移抑制因子促进骨髓中循环B细胞的存活。因此，bmDCs对于维持骨髓中循环B细胞至关重要。
