Pfennig Frank, Kind Barbara, Zieschang Freia, Busch Matthias, Gutzeit Herwig O
Institute of Zoology, TU Dresden, D-01062 Dresden, Germany.
Dev Growth Differ. 2008 Mar;50(3):131-41. doi: 10.1111/j.1440-169X.2008.00986.x. Epub 2008 Feb 27.
A telomerase reverse transcriptase (Tert) encoding gene was cloned from the testis of the teleost fish Oryzias latipes. The expression pattern of Japanese medaka tert (Ola_tert) was analyzed by reverse transcription-polymerase chain reaction and in situ hybridization. Ola_tert was expressed in embryonic stages as well as in differentiated adult tissues. In tissues of adult medakas the highest tert expression was found in gonads and brain. Furthermore, two different splice variants were described and an Ola_tert antisense transcript was identified. The enzyme activity of Tert was determined using a non-radioactive telomeric amplification protocol and the telomerase activity in various tissues was shown to correlate with the tert expression. The telomerase activity was found to be high in contrast to the generally low activity in differentiated human tissues.
从硬骨鱼青鳉的睾丸中克隆出了一个编码端粒酶逆转录酶（Tert）的基因。通过逆转录-聚合酶链反应和原位杂交分析了日本青鳉tert（Ola_tert）的表达模式。Ola_tert在胚胎阶段以及分化的成体组织中均有表达。在成年青鳉的组织中，tert表达量最高的是性腺和脑。此外，还描述了两种不同的剪接变体，并鉴定出了一种Ola_tert反义转录本。使用非放射性端粒扩增方案测定了Tert的酶活性，结果表明不同组织中的端粒酶活性与tert表达相关。与分化的人类组织中普遍较低的活性形成对比的是，端粒酶活性在青鳉中较高。
