Fundación para Investigación Hospital La Fe, Valencia 46009, Spain.
Int J Biol Sci. 2011 Apr 15;7(4):410-7. doi: 10.7150/ijbs.7.410.
To understand the molecular mechanisms that regulate the biology of embryonic stem cells (ESCs) it is necessary to study how they behave in vivo in their natural environment. It is particularly important to study the roles and interactions of the different proteins involved in pluripotency and to use this knowledge for therapeutic purposes. The recent description of key pluripotency factors like Oct4 and Nanog in non-mammalian species has introduced other animal models, such as chicken, Xenopus, zebrafish and medaka, to the study of pluripotency in vivo. These animal models complement the mouse model and have provided new insights into the evolution of Oct4 and Nanog and their different functions during embryonic development. Furthermore, other pluripotency factors previously identified in teleost fish such as Klf4, STAT3, Sox2, telomerase and Tcf3 can now be studied in the context of a functional pluripotency network. The many experimental advantages of fish will fuel rapid analysis of the roles of pluripotency factors in fish embryonic development and the identification of new molecules and mechanisms governing pluripotency.
为了了解调控胚胎干细胞（ESCs）生物学的分子机制，有必要研究它们在其自然环境中体内的行为。研究参与多能性的不同蛋白质的作用和相互作用，并将这些知识用于治疗目的尤为重要。最近在非哺乳动物物种中描述了关键的多能性因子，如 Oct4 和 Nanog，这引入了其他动物模型，如鸡、非洲爪蟾、斑马鱼和青鳉，用于体内多能性的研究。这些动物模型补充了小鼠模型，并为 Oct4 和 Nanog 的进化及其在胚胎发育过程中的不同功能提供了新的见解。此外，现在可以在功能性多能性网络的背景下研究先前在硬骨鱼中鉴定出的其他多能性因子，如 Klf4、STAT3、Sox2、端粒酶和 Tcf3。鱼类的许多实验优势将促进对多能性因子在鱼类胚胎发育中的作用的快速分析，并鉴定出控制多能性的新分子和机制。
