Tabacchioni Silvia, Ferri Lorenzo, Manno Graziana, Mentasti Massimo, Cocchi Priscilla, Campana Silvia, Ravenni Novella, Taccetti Giovanni, Dalmastri Claudia, Chiarini Luigi, Bevivino Annamaria, Fani Renato
ENEA - CRE - CASACCIA -Department of Biotechnologies, Agroindustry, Protection of Health - Plant genetics and Genomics Section, S. Maria di Galeria, Rome, Italy.
FEMS Microbiol Lett. 2008 Apr;281(2):175-82. doi: 10.1111/j.1574-6968.2008.01105.x. Epub 2008 Feb 27.
Bacteria of the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that can cause serious infections in lungs of cystic fibrosis patients. The Bcc comprises at least nine species that have been discriminated by a polyphasic taxonomic approach. In this study, we focused on the gyrB gene, universally distributed among bacteria, as a new target gene to discriminate among the Bcc species. New PCR primers were designed to amplify a gyrB DNA fragment of about 1900 bp from 76 strains representative of all Bcc species. Nucleotide sequences of PCR products were determined and showed more than 400 polymorphic sites with high sequence similarity values from most isolates of the same species. Phylogenetic tree analysis revealed that most of the 76 gyrB sequences grouped, forming clusters, each corresponding to a given Bcc species.
洋葱伯克霍尔德菌复合体（Bcc）细菌是机会致病菌，可在囊性纤维化患者的肺部引起严重感染。Bcc至少包括9个物种，这些物种已通过多相分类方法进行了区分。在本研究中，我们聚焦于普遍存在于细菌中的gyrB基因，将其作为区分Bcc物种的新靶基因。设计了新的PCR引物，以从代表所有Bcc物种的76株菌株中扩增出约1900 bp的gyrB DNA片段。测定了PCR产物的核苷酸序列，结果显示，在来自同一物种的大多数分离株中，有400多个多态性位点，且序列相似性值较高。系统发育树分析表明，76个gyrB序列中的大多数聚在一起，形成簇，每个簇对应一个特定的Bcc物种。
