Cheng Mei-Ling, Chen Chin-Ming, Gu Po-Wen, Ho Hung-Yao, Chiu Daniel Tsun-Yee
Graduate Institute of Medical Biotechnology and Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kwei-san, Tao-yuan, Taiwan.
Clin Biochem. 2008 May;41(7-8):554-60. doi: 10.1016/j.clinbiochem.2008.02.006. Epub 2008 Feb 20.
Inflammation, a major risk factor for acute myocardial infarction (AMI), is associated with leukocytic activation, secretion of myeloperoxidase (MPO) and generation of the oxidative damage marker, 3-chlorotyrosine (3-Cl-Tyr). To study their association with AMI and their value in diagnosis of AMI, white blood cell (WBC) count, plasma MPO, plasma 3-Cl-Tyr, and conventional risk factors such as cardiac troponin I and CK-MB were examined in AMI patients during the onset of chest pain.
After obtaining informed consent, blood samples were collected from 77 AMI patients during the onset of chest pain and from 53 normal controls. The samples were analyzed for WBC count using SE-9000 automated analyzer. Plasma MPO was measured by an enzyme-linked immunosorbent assay. Plasma levels of 3-Cl-Tyr, a product of MPO, were analyzed by HPLC coupled with Coularray electrochemical detection.
The WBC, plasma MPO and 3-Cl-Tyr levels were significantly elevated in AMI patients than in normal controls (p<0.001). The levels of WBC, MPO and 3-Cl-Tyr alone were strongly associated with the prevalence of AMI. Plasma MPO was correlated with 3-Cl-Tyr (r=0.389, p<0.01) and WBC counts (r=0.405, p<0.01) respectively. The ROC curve analyses suggested that MPO had the best specificity and sensitivity among these oxidative stress-related markers.
Plasma MPO value should be considered as a better marker for early diagnosis of AMI, as compared with WBC count or 3-Cl-Tyr.
炎症是急性心肌梗死（AMI）的主要危险因素，与白细胞激活、髓过氧化物酶（MPO）分泌及氧化损伤标志物3-氯酪氨酸（3-Cl-Tyr）的产生有关。为研究它们与AMI的关联及其在AMI诊断中的价值，对胸痛发作时的AMI患者进行了白细胞（WBC）计数、血浆MPO、血浆3-Cl-Tyr以及心肌肌钙蛋白I和肌酸激酶同工酶MB等传统危险因素的检测。
在获得知情同意后，采集了77例胸痛发作时的AMI患者及53例正常对照者的血样。使用SE-9000自动分析仪分析血样中的WBC计数。采用酶联免疫吸附测定法检测血浆MPO。通过高效液相色谱结合库仑阵列电化学检测法分析MPO产物3-Cl-Tyr的血浆水平。
AMI患者的WBC、血浆MPO和3-Cl-Tyr水平显著高于正常对照者（p<0.001）。单独的WBC、MPO和3-Cl-Tyr水平与AMI患病率密切相关。血浆MPO分别与3-Cl-Tyr（r=0.389，p<0.01）和WBC计数（r=0.405，p<0.01）相关。ROC曲线分析表明，在这些氧化应激相关标志物中，MPO具有最佳的特异性和敏感性。
与WBC计数或3-Cl-Tyr相比，血浆MPO值应被视为AMI早期诊断的更好标志物。
