髓过氧化物酶诱导人脐静脉内皮细胞-12凋亡的分子机制。 （注：原文“Olecular”有误，推测应为“Molecular”）
Olecular mechanism underlying the myeloperoxidase induced apoptosis of HUVEC-12 cells.
作者信息
Ouyang Mao, Liu Hengdao, Yang Kan, Jiang Weihong, Ding Qi, Yu Xunzhang, Chen Wu
机构信息
Department of Cardiology, The Third Xiangya Hospital of Central South University Changsha, Hunan 410013, China.
出版信息
Int J Clin Exp Med. 2014 Apr 15;7(4):879-85. eCollection 2014.
OBJECTIVE
This study aimed to investigate the molecular mechanism underlying the myeloperoxidase (MPO) induced apoptosis of human umbilical vein endothelial cells (HUVECs).
METHODS
HUVEC-12 cells were treated with myeloperoxidase at different concentrations (0.1 μ/ml, 0.2 μ/ml, 0.4 μ/ml and 0.6 μ/ml) and apoptotic cells were detected by flow cytometry. Then, cells were harvested for the detection of mRNA and protein expression of caspase-3 and Bax by reverse transcription PCR and Western blot assay, respectively.
RESULTS
When compared with negative control group, the apoptosis index in 0.2 μ/ml MPO group, 0.4 μ/ml MPO group and 0.6 μ/ml MPO group increased markedly (P<0.05). When compared with negative control group, the mRNA expression of caspase-3 in 0.6 μ/ml MPO group and positive control group increased dramatically (P<0.05). When compared with negative control group, the protein expression of pre-caspase-3 and activated caspase-3 elevated significantly in 0.4 μ/ml MPO group, 0.6 μ/ml MPO group and positive control group (P<0.05). When compared with negative control group, the mRNA and protein expression of Bax elevated dramatically in 0.4 μ/ml MPO group, 0.6 μ/ml MPO group and positive control group (P<0.05).
CONCLUSION
MPO at certain extents may induce the apoptosis of HUVEC-12. The MPO induced apoptosis of HUVEC-12 may be dependent on capase-3 signaling pathway, and Bax is also involved in the MPO induced apoptosis of HUVEC-12.
目的
本研究旨在探讨髓过氧化物酶（MPO）诱导人脐静脉内皮细胞（HUVECs）凋亡的分子机制。
方法
用不同浓度（0.1μ/ml、0.2μ/ml、0.4μ/ml和0.6μ/ml）的髓过氧化物酶处理HUVEC-12细胞，通过流式细胞术检测凋亡细胞。然后，分别通过逆转录PCR和蛋白质免疫印迹法检测细胞中半胱天冬酶-3（caspase-3）和Bax的mRNA及蛋白表达。
结果
与阴性对照组相比，0.2μ/ml MPO组、0.4μ/ml MPO组和0.6μ/ml MPO组的凋亡指数显著升高（P<0.05）。与阴性对照组相比，0.6μ/ml MPO组和阳性对照组中caspase-3的mRNA表达显著增加（P<0.05）。与阴性对照组相比，0.4μ/ml MPO组、0.6μ/ml MPO组和阳性对照组中前半胱天冬酶-3和活化的半胱天冬酶-3的蛋白表达显著升高（P<0.05）。与阴性对照组相比，0.4μ/ml MPO组、0.6μ/ml MPO组和阳性对照组中Bax的mRNA和蛋白表达显著升高（P<0.05）。
结论
一定程度的MPO可能诱导HUVEC-12细胞凋亡。MPO诱导HUVEC-12细胞凋亡可能依赖于半胱天冬酶-3信号通路，且Bax也参与了MPO诱导的HUVEC-12细胞凋亡。
Zotero 插件