de Oliveira Edward José, Kanamura Hermínia Yohko, Takei Kioko, Hirata Rosario Dominguez Crespo, Valli Luiz Carlos Pedrosa, Nguyen Nga Yen, de Carvalho Rodrigues Izabel, de Jesus Amélia Ribeiro, Hirata Mário Hiroyuki
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Cj das Químicas, Bloco 17, 05508-900, São Paulo, SP, Brazil.
Trans R Soc Trop Med Hyg. 2008 Apr;102(4):360-6. doi: 10.1016/j.trstmh.2007.11.008.
A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n=23) and chronic (n=30) schistosomiasis, with other parasitoses (n=39) or without parasitic infections (n=100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in kappa concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P=0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.
一组五种合成肽被用作酶联免疫吸附测定（ELISA-Pp）中的抗原基础，用于曼氏血吸虫病的实验室诊断。血清样本取自患有急性血吸虫病（n = 23）和慢性血吸虫病（n = 30）的个体、患有其他寄生虫病的个体（n = 39）或未感染寄生虫的个体（n = 100）。将ELISA-Pp与用于检测IgM（IgM-ELISA）或IgG（IgG-ELISA）的其他免疫酶法以及用于检测IgM抗体的免疫荧光试验（IgM-IFT）进行比较。在血吸虫病组和非血吸虫病组进行检测时，ELISA-Pp的敏感性和特异性分别为86.8%和94.2%。ELISA-Pp与其他血清学方法的比较得出kappa一致性指数在0.59至0.75之间。抗肽IgG抗体评估显示，急性血吸虫病患者的抗体水平高于慢性血吸虫病患者（P = 0.001）。ELISA-Pp显示出令人满意的敏感性和高特异性，可能构成一种用于曼氏血吸虫病实验室诊断的潜在有用方法。
