Adachi H, Fujiwara Y, Ishii N
Life Science Research Center, Lion Corporation, Kanagawa, Japan.
J Gerontol A Biol Sci Med Sci. 1998 Jul;53(4):B240-4. doi: 10.1093/gerona/53a.4.b240.
Protein carbonyl accumulation is an indicator of oxidative damage during aging. The relationship between oxidative stress and protein carbonylation during aging was studied by using a long (age-1) and a short (mev-1) life span mutant of Caenorhabditis elegans. Protein carbonyl concentrations were similar in young adults of both mutants and wild type; however, the subsequent age-dependent accumulation was different with the genotype. The mev-1 mutant (with 50% superoxide dismutase activity) accumulated protein carbonyl at a faster rate than did wild type, whereas the age-1 mutant exhibited no obvious increase except a significant accumulation at the end of extended life span. Exposure to 70% oxygen between the ages of 4 and 11 days caused afar greater accumulation of carbonyl in mev-1 than in wild type, but not in age-1. In addition, rates of aging were enhanced by oxygen in a concentration-dependent fashion. The age-1 mutant was more resistant to, but mev-1 was more sensitive to, such oxygen enhancements of aging than was wild type. These results provide further evidence that oxidative damage is one of the major causal factors for aging in C. elegans, and that the age-1 and mev-1 genes govern resistance to oxidative stress.
蛋白质羰基积累是衰老过程中氧化损伤的一个指标。利用秀丽隐杆线虫的一个长寿（age-1）和一个短寿（mev-1）寿命突变体，研究了衰老过程中氧化应激与蛋白质羰基化之间的关系。两个突变体的年轻成虫以及野生型的蛋白质羰基浓度相似；然而，随后随年龄增长的积累情况因基因型而异。mev-1突变体（超氧化物歧化酶活性为50%）积累蛋白质羰基的速度比野生型快，而age-1突变体除了在延长寿命末期有显著积累外，没有明显增加。在4至11天龄时暴露于70%氧气中，mev-1中羰基的积累比野生型多得多，但age-1中没有。此外，衰老速度以浓度依赖的方式因氧气而加快。与野生型相比，age-1突变体对这种氧气加速衰老更具抗性，但mev-1更敏感。这些结果进一步证明氧化损伤是秀丽隐杆线虫衰老的主要因果因素之一，并且age-1和mev-1基因控制对氧化应激的抗性。
