Funes Soledad, Franzén Lars-Gunnar, González-Halphen Diego
Institut für Physiologische Chemie, Ludwig-Maximilians-Universität München, Germany.
Methods Mol Biol. 2007;372:137-49. doi: 10.1007/978-1-59745-365-3_10.
Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular division, flagellar biogenesis, and, more recently, mitochondrial function. It has distinct advantages in comparison to higher plants because it is unicellular, haploid, and amenable to tetrad analysis, and its three genomes are subject to specific transformation. It also has the possibility to grow either photoautotrophically or heterotrophically on acetate, making the assembly of the photosynthetic machinery not essential for cell viability. Methods developed allow the isolation of C. reinhardtii mitochondria free of thylakoid contaminants. We review the general procedures used for the biochemical characterization of mitochondria from this green alga.
莱茵衣藻是一种用于研究光合作用、细胞分裂、鞭毛生物发生以及最近的线粒体功能的模式生物。与高等植物相比，它具有明显的优势，因为它是单细胞、单倍体，适合进行四分体分析，并且其三个基因组都可以进行特定的转化。它还能够在醋酸盐上进行光合自养或异养生长，这使得光合机器的组装对于细胞活力而言并非必不可少。所开发的方法能够分离出不含类囊体污染物的莱茵衣藻线粒体。我们综述了用于对这种绿藻线粒体进行生化特性分析的一般程序。
