Gandre Shilpa, van der Bliek Alexander M
Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Methods Mol Biol. 2007;372:485-501. doi: 10.1007/978-1-59745-365-3_34.
The study of mitochondrial division proteins has largely focused on yeast and mammalian cells. We describe methods to use Caenorhabditis elegans as an alternative model for studying mitochondrial division, taking advantage of the many wonderful resources provided by the C. elegans community. Our methods are largely based on manipulation of gene expression using classic and molecular genetic techniques combined with fluorescence microscopy. Some biochemical methods are also included. As antibodies become available, these biochemical methods are likely to become more sophisticated.
线粒体分裂蛋白的研究主要集中在酵母和哺乳动物细胞上。我们描述了利用秀丽隐杆线虫作为研究线粒体分裂的替代模型的方法，利用了秀丽隐杆线虫研究群体提供的众多优质资源。我们的方法主要基于使用经典和分子遗传学技术结合荧光显微镜来操纵基因表达。还包括一些生化方法。随着抗体的出现，这些生化方法可能会变得更加成熟。
