拟南芥线粒体蛋白质组学

Arabidopsis mitochondrial proteomics.

作者信息

Heazlewood Joshua L, Millar A Harvey

机构信息

ARC Centre of Excellence in Plant Energy Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, Crawley.

出版信息

Methods Mol Biol. 2007;372:559-71. doi: 10.1007/978-1-59745-365-3_38.

DOI:10.1007/978-1-59745-365-3_38

PMID:18314751

Abstract

Significant efforts have sought to uncover the protein profile of Arabidopsis mitochondria to act as a model for the mitochondrial proteome from plants. A combination of techniques have been undertaken to achieve this goal. We outline a basic two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation of mitochondrial proteins, in-gel trypsination techniques, complex protein lysate digestions, and the identification of proteins by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.

摘要

人们付出了巨大努力来揭示拟南芥线粒体的蛋白质谱，以作为植物线粒体蛋白质组的模型。为实现这一目标，已采用了多种技术的组合。我们概述了线粒体蛋白质的基本二维聚丙烯酰胺凝胶电泳（2D-PAGE）分离、凝胶内胰蛋白酶消化技术、复杂蛋白质裂解物消化，以及通过基质辅助激光解吸/电离（MALDI）和电喷雾电离（ESI）质谱对蛋白质进行鉴定的方法。